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Gene Expression and Transcriptome Analysis
Comprehensive Transcriptome and Gene Expression Analysis
Transcriptome analysis experiments can characterize transcriptional activity (coding and non-coding), focus on a subset of relevant target genes and transcripts, or profile thousands of genes at once to create a global picture of cell function. Gene expression analysis studies can provide a snapshot of actively expressed genes and transcripts under various conditions.
Illumina offers comprehensive next-generation sequencing (NGS) solutions that provide high-quality gene expression and transcriptome analysis data for a broad range of sample types. NGS-based RNA sequencing (RNA-Seq) methods can quantify and profile any active gene or transcript, including novel transcripts.
Gene Expression Profiling with Sequencing
RNA-Seq provides a unique combination of transcriptome-wide coverage, sensitivity, and accuracy for a comprehensive view of gene expression changes. Illumina RNA-Seq solutions provide precise measurement of strand orientation, uniform coverage, and high confidence mapping of alternate transcripts and gene fusions. Discover novel gene isoforms, profile gene expression for select targets of interest, analyze the whole coding transcriptome, and accurately perform transcript abundance and fold-change measurement.
Learn more about:
- mRNA Sequencing: Discover alternative transcripts, gene fusions, and allele-specific expression patterns with a clear, complete view of the coding transcriptome.
- Targeted RNA Sequencing: Select and sequence specific genes or transcripts of interest. Profile expression of dozens to thousands of target genes or transcripts simultaneously.
Empowering Transcriptomics with RNA Sequencing
This ebook discusses genomics applications in gene expression and regulation research. See how workflows can impact study results.
Whole-Transcriptome Analysis with Sequencing
Capture the broad effects of gene expression changes using whole-transcriptome analysis with total RNA sequencing (total RNA-Seq). The method detects both coding and multiple forms of noncoding RNA for a comprehensive view of the entire transcriptome. Learn more about total RNA-Seq.
Whole-Transcriptome Sequencer
Analyze up to 10 transcriptomes in a single run with the NextSeq Series desktop sequencing system.
Gene Panel Finder
Identify sequencing panels that target your genes of interest.
Find the Right Panel
An Alternative to Array-Based Gene Expression Analysis
While gene expression microarrays are effective for identifying the expression of known genes and transcripts, they cannot detect previously unidentified transcripts. In contrast, RNA-Seq offers a comprehensive gene expression profiling solution, allowing researchers to detect both known and novel genes and transcripts in a single assay. Learn more about RNA-Seq advantages over arrays.
From Arrays to mRNA-Seq
Expression Analysis scientists discuss transitioning from microarrays to mRNA-Seq, and describe their comparison studies.
Related Solutions
Cancer Transcriptome Analysis
Profiling gene expression and transcriptome changes with RNA sequencing can aid in understanding tumor classification and progression. Learn more about cancer RNA-Seq.
Microbial Transcriptome Analysis
Bacterial, viral, and other microbial RNA-Seq experiments enable annotation and quantification of comprehensive microbial transcripts. Learn more about microbial RNA-Seq.
Complex Disease Research
Find out how genomic technologies are introducing new avenues for understanding complex disease etiology on a molecular level. Learn more about complex disease genomics.
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Additional Resources
Analyze Gene Expression in Single Cells
Highly sensitive RNA-Seq methods enable gene expression analysis of very low-input samples, even single cells.
RNA-Seq Data Analysis
User-friendly BaseSpace Apps simplify analysis of gene expression data from RNA-Seq studies.
RNA-Seq with NextSeq 500
NextSeq provides the power and versatility needed to address the full range of transcriptome analysis needs.
Illumina NGS Methods Guide
All the information you need, from library preparation to sequencer selection to analysis. Select the best tools for your lab.
Gene Expression Profiling and Validation
TruSeq Targeted RNA Expression Kits offer highly customizable mid- to high-plex gene expression profiling and validation.
RNA-Seq for Gene Expression Analysis
Illumina offers a complete, accessible RNA-Seq workflow solution for gene expression and transcriptome profiling studies.
Sequencing Method Explorer
Use this interactive tool to explore experimental NGS library preparation methods compiled from the scientific literature.